Topic: CELL-DYN RUBY
Picture taken from: http://www.abbottdiagnostics.com/Products/Instruments_by_Platform/default.cfm?system=cell-dyn&suffix=ruby
HEY people!How's your SIP?I hope its going great aite. As for me, I was attached to the Haematology Lab for the first 2 weeks. Most of the tests there are done by analysers which makes the job of a Medical Technologist much easier. However, Medical Technologist is still responsible for the proper sorting out of the samples and maintaining the machinery so that the result obtain would be accurate. Here is one of the analyser that I use to perform Full Blood Count(FBC).
CELL-DYN RUBY
The CELL-DYN RUBY is use to perform full blood count and retics. It uses the optical technology which is Multi-Angle-Polarized Scatter Separation (MAPSS). It helps to differentiate the different white blood cells and at the same time also measure the full blood count such as MCT, MCV, Hb, WBC, RBC and etc.
Upon receiving EDTA blood sample, checking of clot in the blood is done manually before the EDTA sample is placed into the CELL-DYN RUBY. EDTA sample is checked to see if clot is present because in presence of clot, it will affect the overall full blood count. If blood is found to be clotted, the sample is rejected and a request for a new EDTA blood sample is done. Sometimes, blood are clotted in EDTA tube because it has not been mixed thoroughly. Wherelse for sample that are not clotted, they are placed into a rack which will then enter the CELL-DYN RUBY. Results will directly be uploaded into the LISS.
As for retics, the amount of reticulocytes are counted. The test is slightly different from FBC. It requires prior preparation before loading it in the analyser. 20microlitres of the sample is added to the reticulocyte reagent tube and mixed well. It is then incubated before it is run. Incubation should be between the range of 15minutes to 2hours.
In the laboratory that I am attach to, there are two of the same analysers. Which means there are 2 CELL-DYN RUBY. Two analysers are use together at the same time so to ensure that the turnaround time is shorter. Maintainence of analyser are done once a week. Since there are two analysers running at the same time, correlation is done twice daily. Once in the morning and the other in the evening. Correlation is done by using the same EDTA sample and running it in both machine. Since it is the same EDTA sample, both machine will have to produce similar results to indicate that they are working properly and according to standards. This is also part of the maintainence of the machine. Autovalidation is also done. Every 10 samples, a random sample is collected and result of that random sample is printed form LISS. Peripheral blood film is done and stained. It is then viewed under microscope to manually count and observe the cells. This is to ensure that the analyser is working well.
I hope with this information you guys have learnt and now know another brilliantly invented analyser that makes life of a Medical Technologist easier. Good Luck for your coming weeks!!!
Peace,
Dyana.
11 comments:
Hi dyana,
May I know why is there a need to incubate the blood sample before it is run for retics by the analyser? Over here, we do not need to incubate before putting the sample into the analyser for retics.
Lastly, I’d like to know what exactly is in the retic reagent tube? Why add 20microlitres instead of 25 or 30? Is it because there is something already in the tube before u add the blood sample?
Thanks! =)
Malerie
TG02
Hi dyana,
If the sample are not clotted they are placed into a rack and test directly. Do you need to process the blood before testing? What are the things to take note of other then blood clot?
Thanks
Justina
0605950E
TG01
Hey dy..
Why is there a need for prior preparation before loading the reticulocytes in the analysers for retic count?
Andika Putra
TG01
Hey
You mentioned that the incubation time is 15mins to 2hours. thats a really long incubation period.
is there a criteria to which you have to incubate longer, or shorter? and what happens if you incubate shorter? or longer? cuase i sure dont wanna be incubating all my samples at 2hrs for ideal results.
thanks
GLAD
What happens if there is presence of small blood clots present in the blood tube? Will there be any system or anyone be able to know? Will it badly affect the blood test results?
Hi!
How do you ensure that the blood in the EDTA tube is mixed thoroughly? And .. Is the reticulocyte reagent coated on the tube?
Thanks.
Lyn
TG02
Hi all, sorry for the late reply..
To LYN: To ensure that the EDTA(anticoagulant) is mixed well with the blood sample, once blood is drawn from a patient and place into a tube containing EDTA, the tube is to be fully inverted for about 5 times. Some the nurses or phebetolmist fail to do so and that causes the blood to clot.
The CELL-DYN reticulocyte reagent contains phosphate buffered saline solution containing new methylene blue, potassium oxalate and n-dodecyl B-D-maltoside. It usually comes in 100tubes (in a box) and each tube is 3.7mL each containing all the substance above.Every request for a rectic test only requires one tube.
To MS CHEW: The medical technologist in the hematology section will receive the blood from the processing section, they will then check for blood clot manually using a stick applicator (looks like satay stick but not sharp) to dig out any clot if present. If blood clot is present, the medical technologist will have to cancel the current test and request for a new blood sample.
And yes, it will affect the result as blood components are clotted and the measurement would be inaccurate.
To GLAD: The medical technologist there will do a standard incubation of 15minutes to ensure that the blood sample is mixed well with the reticulocyte reagent. From the manual procedure, the incubation time cannot exceed more than 2hours. If it does, then it has to be discard.
Hey ANDIKA: We have to make prior preparation as in pipet 20 microlitres of the whole blood specimen(from the patient sample) into the CELL-DYN reticulocyte reagent . Then the tube is fully inverted for few times before incubating it for 15mins so that the sample will mix with the reagent. Reticulocytes(immature RBCs) contains ribosomal RNA, thus RNA can be seen with certain supravital, cationic dyes that stimultaneously stain and precipitate the polyanion to form network or reticulum.
Hey JUSTINA: Process blood before testing? hmm..What they do once they receive the blood sample from the processing section is to firstly check for blood clot and check what test was requested for. If FBC test is requested, then the tube will be placed in a rack and run in the CELL-DYN RUBY analyser.
Hey MALERIE: Incubation is basically to mixed the blood sample with the reticulocyte reagent. Maybe the lab that you're attach to does not use the same analyser? The reason they mixed the blood is because reticulocytes(immature RBCs) contains ribosomal RNA, thus RNA can be seen with certain supravital, cationic dyes that stimultaneously stain and precipitate the polyanion to form network or reticulum.
The CELL-DYN reticulocyte reagent contains phosphate buffered saline solution containing new methylene blue, potassium oxalate and n-dodecyl B-D-maltoside. As for why it is 20microlitres of whole blood specimen is because that is the staining procedure set by the Abbot Laboratories that are the manufacturers of the CELL-DYN RUBY.
Thank You!
Dyana
0605169B
how does clotted blood affect the FBC? increase or decrease? what are the other problems that may arise when u collect the samples?
yuxuan
TO YUXUAN: It will affect the result of the FBC. Since the blood is clotted, it will probably decrease the actually measurement, eg. the platelets count maybe decrease due to the clot.
Nope, the only problem would be the blood clot. At least that's what they told me and that's the only problem i've encountered.
Thank you!
Dyana
0605169B
hey dy..
juz wondering about the retic count. You say that you would run it in the machine right? Does that mean you dun need to manually count the retic under the microscope?? Bcoz at my attachment place, we do the retic count manually..
Sofie
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