xin yi-
so far, i believe that it has been a pretty smooth-sailing ride on my part. nothing lethal whatsoever. haha. though initially, i had a minor issue on adapting to the working environment and understanding the interpersonal dynamics. but time has truly rectify my concerns.
well, if the large tissue is not properly orientated(meaning not of the same level), the tissue block has to be shaved deeper so that a full face can be achieved. however, if the thinner tissues are not embedded to the paraffin wax at the same level, they will have to be re-blocked. this is because shaving the thinner tissues more will risk losing the patient's tissue.
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~immortals~ said... olla!!!!
aint it abit gruesome to see all those parts of the body??
anyway, yew saed that orientation of the tissues are crucial, such as the orientation of the cyst.
so my question is, why is the cyst wall the main interest?
and why must the cyst wall be at the base of the mold, and not be anywhere else?
mayafirhana
tg02
maya-
haha initially, it put me off a bit. but after a while, i am pretty much accustomed to it. A cyst is an abnormal, sac-like structure within a tissue that can contain fluid, air, or semisolid substance. it can be present at anywhere in the body and varies in size. The outer portion of a cyst is termed the cyst wall. i believe that the cyst wall is the most crucial part to produce a diagnosis. the cells at the wall will show if it is benign or malignant. oh, it has to be at the base of the mold because if you recall embedding right, you will always section the surface of the tissue which is at the base of the mold. do you recall? hehe =)
- ~immortals~ said...
Hi Tira!!
U mention that the specimens for surgery are sent to the specimen recieving room. How long are they usually kept there before being processed?
Another quest: What are the possible errors that might arise in the embedding process?
TG02
Amir-
hello Amir!! Specimens FROM surgery are sent to the receiving room to be correlated with the forms. subsequently, they are sent to the trimming room. depending on their sizes, they are either trimmed by the pathologists or immediately placed into the cassettes and fixed in formalin. and after that, they are placed in the processor. so the duration from being in the receiving room to being processed varies. larger tissues, longer duration. hmm errors during embedding..when the paraffin wax has not completely cooled down and it is immediately being sectioned, the tissue will break. that's when re-blocking has to be done.
- tg01 group 2 said...
Hi Athirah,
Some questions to ask you,
1)You mentioned that the forcep is hot and I understand it is sterile.
Wouldn't the heat from the forcep breaks the tissue?
2)What do you use to press the tissue flat down on the mold and how do you ensure this is done properly?
Thankz!
Han Yang
TG01
hey han yang,
1) oh no. it would not. the temperature of the forceps is about 65-70 degrees celsius. At this temperature, the tissue will not break. The heated tips of the forceps actually helps to prevent the tissue from sticking to the tip and hence prevents carry over which could lead to contamination.
2)we use forceps and a flat-surfaced tip made of pure metal.
- BMT said...
Athirah!
Ok I'll bug you with a question. Which I'm sure will be arguably easy for you to answer since you seem to be a pro at Histo already. =P
Anyway, must all tissue samples (like you have mentioned the cyst) be placed at the base, or can they be placed in the center?
Elyana
0606676E
TG01
dear elyn-
it has to be placed at the base so that sectioning can be done efficiently. imagine if it is placed at the center of the mold, the process of sectioning will be prolonged.
- ~immortals~ said...
miss nurathirah,
You mentioned the use of an electrically heated forceps for the transfer of the tissues into the mold.
I would like to know at which temperature are the forceps heated to, as i believe that a temperature which is too high may damage the tissues.
I would greatly appreciate if you could enlighten me on this matter.
thanks!!!!
fellow med tech,
rusydiana
Rusyyy-
the temperature of the forceps is about 65-70 degree celsius. so far i have not witnessed any tissue damage at this temperature. also the electronic forceps have overheat protection, to ensure that the temperature of the forceps do not go beyond 70 degree celsius.
- BMT said...
Hi:) can i ask u if the machine used to fill the wax is the same as the one we have in our sch lab?thanks:) because i am curious how does it fills the wax:)
Rachael
Tg01
hello there rachael-
honestly, i can't remember exactly how the embedding machine in school looks like. haha. well since there are many types of embedding machine. however, i can assure you they consist of the same parts; the wax dispenser, the cold plate, the hot surface, the mold storage area.
i'd appreciate it if you'll correct me if i'm wrong. thanks.
Nurathirah
Tg01
Hi,
So far in your attachment, have you met any difficulties? If there is, can you share?
By the way, you mention that if the orientation of the tissue is not done properly, then it will hinder the sectioning. So what happen if this really happens? Does your lab redo it or just leave it as it is? The samples obtained are limited right?
Thanks. That's all.
Xin Yi
TG02